The TS Absorbance Reader offers both affordability and proven performance for microplate-based assays in in a variety of research and clinical applications. The ELx™ Absorbance Microplate Reader offers proven performance for many applications, from endpoint to fast kinetic protocols. The ELx is widely. ELx Absorbance Microplate Reader with computer software is in our new catalogue Features.
|Language:||English, Spanish, German|
|Distribution:||Free* [*Sign up for free]|
The ELx™ is a compact, robust microplate reader ideally Software, the ELx applications are expanded to include kinetic Absorbance Test Plate. Shop online for a wide selection of BioTek ELx Absorbance Microplate Readers Store up to 75 custom assay definitions and eight test results. 1 Introducing the ELx Absorbance Microplate Reader. and provides the means for downloading additional assay definition files to the instrument.
Accuracy and precision of the dispensed volumes are measured against user-defined tolerance limits and failing values are flagged in the report. Summary statistics by well, by row, and by column are also contained in the summary report. Artel has designed the MVS to be universally applicable to virtually any liquid handler. Currently, any 1-, 2-, 4-, 8-, , , , and channel device, such as handheld pipettes or automated liquid handlers, may be checked for volume dispensing performance.
These devices must be capable of dispensing into a standard or well microtiter plate. The MVS is sold as a complete system.
The system components include a customized Plate Reader, a unique Calibrator Plate, a microtiter Plate Shaker, a bar code reader, a portable computer with the MVS Data Manager system software, a mobile workstation and a user guide. On-site system installation and validation are also included with an MVS purchase. Can I use my own microwell plate reader?
No, we do not support the use of other plate readers at this time. The MVS includes a mobile workstation for convenient verification of equipment in multiple locations throughout a laboratory or facility. Yes, the MVS is a convenient way to verify the accuracy and precision performance of each independent channel at the same time.
The MVS allows for analysis of accuracy and precision for each independent dispensing channel simultaneously, which saves labor and liquid handler down-time. It is relatively immune to environmental conditions and provides measurements traceable to national and international standards.
There is no need to generate standard curves.
MVS provides standardized results making it possible to compare instrument performance between makes and models of liquid handlers as well as between locations, i. The MVS results are traceable to national and international standards. The only regular maintenance required is the recertification of the MVS Calibrator Plate, which has a shelf-life of 1-year. At the one year mark, the Calibrator Plate must be shipped back to Artel for the recertification process.
Aside from Calibrator Plate recertification, the only other routine maintenance required is the occasional replacement of the lamp in the Artel Plate Reader dependent on plate reader used , which can be easily completed by the user.
The MVS can be easily used by technicians and scientists of any education level. Artel has done all the work for liquid handler QC to make volume verification fast, easy, and reliable. MVS Data Manager system software guides the user through the entire volume verification process and all the Sample Solutions are prepared by Artel.
The user simply pours the solutions into a reservoir, dispenses the solution with the liquid handler under test, and measures the tip-by-tip volume values using the MVS. Such sources may include various forms of life like plants, marine macro-organisms sponge, corals and algae and endophytes fungi, bacteria and actinomycetes [ 3 ].
Plants which are used in traditional medicines are of significant importance and therefore considerable research has been carried out on medicinal plants for bioactive compounds however limited research has been performed on the associated microorganisms.
Endophytes are chemical synthesizer inside plants. They play a role as a selection system for microbes to produce pharmacologically active substances with low toxicity toward mammalians [ 4 ]. Endophytes are sometimes responsible for the medicinal properties of their hosts.
Many endophytes synthesize bioactive compounds that can be used by plants for defense against pathogens and some of these compounds may be valuable as pharmaceutical drugs [ 5 ]. Fagonia indica is a small spiny shrub that belongs to the family Zygophyllaceae present in warm and arid regions of the world, especially Asia and Africa [ 6 ].
Fagonia indica is of great ethno pharmacological importance with multiple therapeutic activities such as antimicrobial, antioxidant, antiseptic, anticancer, antidiabetic and anti-inflammatory [ 7 ].
The plant is also used for the treatment of fever, thirst, vomiting, asthma, urinary discharge, dysentery, liver and stomach trouble, toothache, typhoid and skin diseases [ 8 ]. This study was designed to investigate the role of endophytic bacteria in the medicinal properties of F.
Bacteria were isolated, identified and screened for the production of bioactive secondary metabolites. Methods Plants collection and identification The plant samples were collected in sample bags from village Mullazai, Peshawar, Khyber PakhtunKhwa, Pakistan and brought to the laboratory at the same day for the isolation of endophytic bacteria. The plant material was taxonomically identified as F.
Herbarium specimen Voucher No. Isolation of endophytic bacteria from stem of Fagonia indica The stem of the plant was cut into pieces of about 0. Stem pieces 5—6 were blotted on the blotting paper [ 9 ] and placed on selected Tryptic Soy Agar TSA media for the isolation of endophytic bacteria.
Molecular identification of isolated endophytic bacteria Bacterial genomic DNA was isolated using alkaline lysis method and 16S rRNA gene was amplified by performing colony PCR using universal bacterial primers 27F and R, which produced a product of size base pairs [ 10 ].
Sequence results obtained were compared with nucleotide sequences available on NCBI database www. The supernatant was separated in the tube and the pellet was processed further. Pellet was dissolved in organic solvent methanol and incubated for 1 day. Supernatant was separated in the falcon tube B. The pellet was discarded and both the solvents A and B were mixed. The solvent was evaporated at room temperature to get crude extract of bioactive metabolites.
The extract was dissolved in dimethyl sulfoxide DMSO [ 11 ]. The antioxidant potential of the crude extracts was gauged by monitoring its capacity to quench the stable 2, 2-diphenyl 1-picrylhydrazyl DPPH free radical.
Activated crude extract samples of bacterial endophytes i. DMSO and ascorbic acid were taken as negative and positive controls, respectively. After incubation, samples had been cooled at room temperature and transferred to 96 well plates.
DMSO and ascorbic acid were taken as negative and positive controls, respectively and absorbance was measure at using microplate reader ELx BioTek.
The experiment was performed in triplicate. Total reducing power estimation The method described by Oyaizu et al. DMSO and ascorbic acid were used as negative and positive controls, respectively.
Antimicrobial activity Antifungal assay Triplicate analysis by disc diffusion method was used to evaluate the antifungal potential of test extracts described by Lai et al.